shCREATE

A research-use workspace for designing siRNA and shRNA candidates, checking transcript-level off-targets, and preparing order-ready shRNA oligos.

Design Generate candidate oligos around a target sequence position with GC, length, run, and motif filters.
BLAST filter Screen candidates against transcript hits using oligo coverage, mismatch, and E-value cutoffs.
Finalise Export selected IDs, summaries, BLAST tables, and shRNA oligos for downstream review.

shCREATE

siRNA and shRNA design workspace

Ready
0 candidates
ID Sequence Start End Length GC
0 selected IDs
ID Gene hits under cutoff Status
0 final designs
ID Target sequence Reverse complement shRNA fwd shRNA rev Length GC Score